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Image Search Results
Journal: The Journal of investigative dermatology
Article Title: TGFbeta1-induced inflammation in premalignant epidermal squamous lesions requires IL-17.
doi: 10.1038/jid.2010.92
Figure Lengend Snippet: Figure 2. TGFb1 induces IL-17 production in CD4 þ and cd þ tumor-infiltrating T cells. Tumor-infiltrating lymphocytes were isolated from papillomas after 2 days of dox treatment and analyzed for cell-surface markers and intracellular cytokines. (a) A representative flow cytometry profile of IL-17 þ and IFNg þ T cells from ST and DT papillomas. (b) Percentage of IL-17 þ and IFNg þ CD4 þ T cells from ST (n ¼ 11) and DT (n ¼ 12) papillomas, and IL-17 þ
Article Snippet: For immunohistochemistry, anti-human MPO (Dako, Carpinteria, CA); anti-CD3e (Santa Cruz Biotechnology, Santa Cruz, CA);
Techniques: Isolation, Flow Cytometry
Journal: The Journal of investigative dermatology
Article Title: TGFbeta1-induced inflammation in premalignant epidermal squamous lesions requires IL-17.
doi: 10.1038/jid.2010.92
Figure Lengend Snippet: Figure 4. TGFb1 overexpression increases inflammatory infiltrate in benign tumors. ST papillomas (a, c) and DT papillomas (b, d–f) were harvested from mice placed on dox for 1 week. Tumors were stained with hematoxylin and eosin-Y (a, b), antiMPO for myeloid cells (c, d), and anti-CD3e (e) and anti-CD8 (f). Bars ¼ 20 mM. The arrows indicate infiltrating leukocytes. The photomicrographs are representative of at least three different tumors per genotype. S, stroma; T, tumor. (g–i) Quantitation of tumor-infiltrating T cells from immunohistochemical analysis for CD3 þ (g), CD4 þ (h), and CD8 þ (i) cells. No staining was detected in the absence of primary antibody. Each histogram represents the average from at least four tumors of each genotype, with more than five fields counted per section. Error bars ¼ ±SEM.
Article Snippet: For immunohistochemistry, anti-human MPO (Dako, Carpinteria, CA); anti-CD3e (Santa Cruz Biotechnology, Santa Cruz, CA);
Techniques: Over Expression, Staining, Quantitation Assay, Immunohistochemical staining